BD HorizonTM PE-CF594 Mouse Anti-Human CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 expression by resting and activated human leucocytes. Panels 1 and 2. CD279 PD-1 expression on resting peripheral blood leucocytes. Fresh whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either BD Horizon PE-CF594 Mouse IgG1 kappa Isotype Control Cat. No. 562292; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566845/566846; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus side-light scatter SSC-A signals Panel 1 or CD3 Panel 2 were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Panel 3. CD279 PD-1 expression on stimulated peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon PE-CF594 Mouse IgG1 kappa Isotype Control dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human CD279 PD-1 antibody solid line histogram . The fluorescence histogram showing CD279 PD-1 expression or Ig Isotype control staining was derived from gated events forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD279 PD-1 expression by resting and activated human leucocytes. Panels 1 and 2. CD279 PD-1 expression on resting peripheral blood leucocytes. Fresh whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either BD Horizon PE-CF594 Mouse IgG1 kappa Isotype Control Cat. No. 562292; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566845/566846; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus side-light scatter SSC-A signals Panel 1 or CD3 Panel 2 were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Panel 3. CD279 PD-1 expression on stimulated peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon PE-CF594 Mouse IgG1 kappa Isotype Control dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human CD279 PD-1 antibody solid line histogram . The fluorescence histogram showing CD279 PD-1 expression or Ig Isotype control staining was derived from gated events forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD279 PD-1 expression by resting and activated human leucocytes. Panels 1 and 2. CD279 PD-1 expression on resting peripheral blood leucocytes. Fresh whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either BD Horizon PE-CF594 Mouse IgG1 kappa Isotype Control Cat. No. 562292; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566845/566846; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus side-light scatter SSC-A signals Panel 1 or CD3 Panel 2 were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Panel 3. CD279 PD-1 expression on stimulated peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon PE-CF594 Mouse IgG1 kappa Isotype Control dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human CD279 PD-1 antibody solid line histogram . The fluorescence histogram showing CD279 PD-1 expression or Ig Isotype control staining was derived from gated events forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.