BD HorizonTM PE-CF594 Mouse Anti-Human CD69

Description

Flow cytometric analysis of CD69 expressed on stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype control Cat. No. 562292; dashed line histogram or with the BD Horizon PE-CF594 Mouse Anti-Human CD69 antibody Cat. No. 562617/562645; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable activated lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD69 expressed on stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype control Cat. No. 562292; dashed line histogram or with the BD Horizon PE-CF594 Mouse Anti-Human CD69 antibody Cat. No. 562617/562645; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable activated lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD69 expressed on stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype control Cat. No. 562292; dashed line histogram or with the BD Horizon PE-CF594 Mouse Anti-Human CD69 antibody Cat. No. 562617/562645; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable activated lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.