BD HorizonTM PE-CF594 Mouse Anti-Human CD69

Description

Flow cytometric analysis of CD69 expression on stimulated Rhesus peripheral blood mononuclear cells. Rhesus peripheral blood mononuclear cells were stimulated overnight with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or with the BD Horizon PE-CF594 Mouse Anti-Human CD69 antibody Cat. No. 562645/562617; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD69 expression on stimulated Rhesus peripheral blood mononuclear cells. Rhesus peripheral blood mononuclear cells were stimulated overnight with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or with the BD Horizon PE-CF594 Mouse Anti-Human CD69 antibody Cat. No. 562645/562617; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD69 expression on stimulated Rhesus peripheral blood mononuclear cells. Rhesus peripheral blood mononuclear cells were stimulated overnight with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or with the BD Horizon PE-CF594 Mouse Anti-Human CD69 antibody Cat. No. 562645/562617; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.