
565682 ·
BDB Bioscience · Cat: 565682
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human Galectin-3 antibody Cat. No. 565682; solid line histogram at 1.0 ug/test. The fluorescence histogram showing Galectin-3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data on this Technical Data Sheet is not lot specific. Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human Galectin-3 antibody Cat. No. 565682; solid line histogram at 1.0 ug/test. The fluorescence histogram showing Galectin-3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data on this Technical Data Sheet is not lot specific. Show More Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human Galectin-3 antibody Cat. No. 565682; solid line histogram at 1.0 ug/test. The fluorescence histogram showing Galectin-3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data on this Technical Data Sheet is not lot specific.