BD HorizonTM PE-CF594 Mouse Anti-Human Granzyme B

Description

Multicolor flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with FITC Mouse Anti-Human CD8 Cat. No. 555366/561947/561948 and either BD Horizon PE-CF594 Mouse IgG1 Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Granzyme B antibody Cat. No. 562462; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Mouse IgG1 Isotype Control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with FITC Mouse Anti-Human CD8 Cat. No. 555366/561947/561948 and either BD Horizon PE-CF594 Mouse IgG1 Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Granzyme B antibody Cat. No. 562462; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Mouse IgG1 Isotype Control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with FITC Mouse Anti-Human CD8 Cat. No. 555366/561947/561948 and either BD Horizon PE-CF594 Mouse IgG1 Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Granzyme B antibody Cat. No. 562462; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Mouse IgG1 Isotype Control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with FITC Mouse Anti-Human CD8 Cat. No. 555366/561947/561948 and either BD Horizon PE-CF594 Mouse IgG1 Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Granzyme B antibody Cat. No. 562462; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Mouse IgG1 Isotype Control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with FITC Mouse Anti-Human CD8 Cat. No. 555366/561947/561948 and either BD Horizon PE-CF594 Mouse IgG1 Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Granzyme B antibody Cat. No. 562462; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Mouse IgG1 Isotype Control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with FITC Mouse Anti-Human CD8 Cat. No. 555366/561947/561948 and either BD Horizon PE-CF594 Mouse IgG1 Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Granzyme B antibody Cat. No. 562462; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of Granzyme B or Mouse IgG1 Isotype Control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.