BD HorizonTM PE-CF594 Mouse Anti-Human IgM

Description

Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD Horizon PE-CF594 Mouse Anti-Human IgM Cat. No. 562539 and APC Mouse Anti-Human CD19 Cat. No. 555415/561742 antibodies. A two-color flow cytometric dot plot showing the correlated expression of cell surface IgM versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD Horizon PE-CF594 Mouse Anti-Human IgM Cat. No. 562539 and APC Mouse Anti-Human CD19 Cat. No. 555415/561742 antibodies. A two-color flow cytometric dot plot showing the correlated expression of cell surface IgM versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD Horizon PE-CF594 Mouse Anti-Human IgM Cat. No. 562539 and APC Mouse Anti-Human CD19 Cat. No. 555415/561742 antibodies. A two-color flow cytometric dot plot showing the correlated expression of cell surface IgM versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.