BD HorizonTM PE-CF594 Mouse Anti-Human Ikaros

Description

Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Ikaros antibody Cat. No. 564866; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Ikaros antibody Cat. No. 564866; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Ikaros antibody Cat. No. 564866; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Ikaros antibody Cat. No. 564866; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Ikaros antibody Cat. No. 564866; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human Ikaros antibody Cat. No. 564866; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.