BD HorizonTM PE-CF594 Mouse Anti-Human IL-8

Description

Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human IL-8 antibody Cat. No. 563531; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human IL-8 antibody Cat. No. 563531; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human IL-8 antibody Cat. No. 563531; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human IL-8 antibody Cat. No. 563531; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human IL-8 antibody Cat. No. 563531; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Panel or BD Horizon PE-CF594 Mouse Anti-Human IL-8 antibody Cat. No. 563531; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.