BD HorizonTM PE-CF594 Mouse Anti-Human NCAM-1 (CD56)

Description

Two-color flow cytometric analysis of NCAM-1 CD56 expression by fixed and permeabilized human peripheral blood lymphocytes. Erythrocytes were lysed and leucocytes were fixed and permeabilized in a human whole blood sample using 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C followed by BD Phosflow Perm Buffer III Cat. No. 558050; 30 min, on ice . The leucocytes were then stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 Cat. No. 564963 and BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 antibodies. The two-color flow cytometric dot plot showing the correlated expression of NCAM-1 CD56 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of NCAM-1 CD56 expression on live-stained human peripheral blood lymphocytes. Whole blood was stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 Cat. No. 564963 and BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 antibodies. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric dot plot showing the correlated expression of NCAM-1 CD56 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of NCAM-1 CD56 expression by fixed and permeabilized human peripheral blood lymphocytes. Erythrocytes were lysed and leucocytes were fixed and permeabilized in a human whole blood sample using 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C followed by BD Phosflow Perm Buffer III Cat. No. 558050; 30 min, on ice . The leucocytes were then stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 Cat. No. 564963 and BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 antibodies. The two-color flow cytometric dot plot showing the correlated expression of NCAM-1 CD56 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of NCAM-1 CD56 expression on live-stained human peripheral blood lymphocytes. Whole blood was stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 Cat. No. 564963 and BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 antibodies. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric dot plot showing the correlated expression of NCAM-1 CD56 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of NCAM-1 CD56 expression by fixed and permeabilized human peripheral blood lymphocytes. Erythrocytes were lysed and leucocytes were fixed and permeabilized in a human whole blood sample using 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C followed by BD Phosflow Perm Buffer III Cat. No. 558050; 30 min, on ice . The leucocytes were then stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 Cat. No. 564963 and BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 antibodies. The two-color flow cytometric dot plot showing the correlated expression of NCAM-1 CD56 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of NCAM-1 CD56 expression on live-stained human peripheral blood lymphocytes. Whole blood was stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 Cat. No. 564963 and BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 antibodies. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric dot plot showing the correlated expression of NCAM-1 CD56 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.