BD HorizonTM PE-CF594 Mouse Anti-Human STING

Description

Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD20 antibody Cat. No. 558054 and either BD Horizon PE-CF594 IgG1, kappa Isotype Control Cat. No. 562292; Top Panels or BD Horizon PE-CF594 Mouse Anti-Human STING antibody Cat. No. 565313; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD20 antibody Cat. No. 558054 and either BD Horizon PE-CF594 IgG1, kappa Isotype Control Cat. No. 562292; Top Panels or BD Horizon PE-CF594 Mouse Anti-Human STING antibody Cat. No. 565313; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD20 antibody Cat. No. 558054 and either BD Horizon PE-CF594 IgG1, kappa Isotype Control Cat. No. 562292; Top Panels or BD Horizon PE-CF594 Mouse Anti-Human STING antibody Cat. No. 565313; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC-A were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.