BD HorizonTM PE-CF594 Mouse Anti-Human TCR Vbeta13.1

Description

Multiparameter flow cytometric analysis of TCR Vbeta13.1 expression on Human peripheral blood T lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with BD Horizon BV421 Mouse Anti Human CD3 antibody Cat. No, 562426; Upper Plots and with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human TCR Vbeta13.1 antibody Cat. No. 569816/569890; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of TCR Vbeta13.1 or Ig Isotype control staining versus CD3 Upper Plots or side-light scatter SSC-A signals Lower Plots was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of TCR Vbeta13.1 expression on Human peripheral blood T lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with BD Horizon BV421 Mouse Anti Human CD3 antibody Cat. No, 562426; Upper Plots and with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human TCR Vbeta13.1 antibody Cat. No. 569816/569890; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of TCR Vbeta13.1 or Ig Isotype control staining versus CD3 Upper Plots or side-light scatter SSC-A signals Lower Plots was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of TCR Vbeta13.1 expression on Human peripheral blood T lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with BD Horizon BV421 Mouse Anti Human CD3 antibody Cat. No, 562426; Upper Plots and with either BD Horizon PE-CF594 Mouse IgG2b, kappa Isotype Control Cat. No. 562305; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human TCR Vbeta13.1 antibody Cat. No. 569816/569890; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of TCR Vbeta13.1 or Ig Isotype control staining versus CD3 Upper Plots or side-light scatter SSC-A signals Lower Plots was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.