BD HorizonTM PE-CF594 Mouse Anti-Human VISTA

Description

Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292`; Left Plot or BD Horizon PE-CF594 Mouse Anti-Human VISTA antibody Cat. No. 566772/566773; Right Plot . The two-parameter flow cytometric contour plot showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292`; Left Plot or BD Horizon PE-CF594 Mouse Anti-Human VISTA antibody Cat. No. 566772/566773; Right Plot . The two-parameter flow cytometric contour plot showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292`; Left Plot or BD Horizon PE-CF594 Mouse Anti-Human VISTA antibody Cat. No. 566772/566773; Right Plot . The two-parameter flow cytometric contour plot showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292`; Left Plot or BD Horizon PE-CF594 Mouse Anti-Human VISTA antibody Cat. No. 566772/566773; Right Plot . The two-parameter flow cytometric contour plot showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292`; Left Plot or BD Horizon PE-CF594 Mouse Anti-Human VISTA antibody Cat. No. 566772/566773; Right Plot . The two-parameter flow cytometric contour plot showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of VISTA expression on human peripheral blood leucocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then washed and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292`; Left Plot or BD Horizon PE-CF594 Mouse Anti-Human VISTA antibody Cat. No. 566772/566773; Right Plot . The two-parameter flow cytometric contour plot showing the correlated expression of VISTA or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.