BD HorizonTM PE-CF594 Mouse Anti-Mouse CD366 (TIM-3)

Description

Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432; 1 mug/ml antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and then stained with BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566998; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432; 1 mug/ml antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and then stained with BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566998; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432; 1 mug/ml antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and then stained with BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566998; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432; 1 mug/ml antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and then stained with BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566998; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432; 1 mug/ml antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and then stained with BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566998; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432; 1 mug/ml antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and then stained with BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898 and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 566998; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD8a versus CD366 TIM-3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.