BD HorizonTM PE-CF594 Mouse Anti-T-bet

Description

Flow cytometric analysis of T-bet expression in human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with Alexa Fluor 647 Mouse Anti-Human CD3 antibody Cat. No. 557706 and with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-T-bet antibody Cat. No. 562467, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of T-bet or Ig isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of T-bet expression in human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with Alexa Fluor 647 Mouse Anti-Human CD3 antibody Cat. No. 557706 and with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-T-bet antibody Cat. No. 562467, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of T-bet or Ig isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of T-bet expression in human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with Alexa Fluor 647 Mouse Anti-Human CD3 antibody Cat. No. 557706 and with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-T-bet antibody Cat. No. 562467, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of T-bet or Ig isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of T-bet expression in human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with Alexa Fluor 647 Mouse Anti-Human CD3 antibody Cat. No. 557706 and with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-T-bet antibody Cat. No. 562467, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of T-bet or Ig isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of T-bet expression in human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with Alexa Fluor 647 Mouse Anti-Human CD3 antibody Cat. No. 557706 and with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-T-bet antibody Cat. No. 562467, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of T-bet or Ig isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of T-bet expression in human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with Alexa Fluor 647 Mouse Anti-Human CD3 antibody Cat. No. 557706 and with either a BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panel or BD Horizon PE-CF594 Mouse Anti-T-bet antibody Cat. No. 562467, Right Panel . Two-color flow cytometric dot plots showing the correlated expression of T-bet or Ig isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.