BD HorizonTM PE-CF594 Rat Anti-Mouse CD150 (SLAM)

Description

Multicolor flow cytometric analysis of CD150 SLAM expression by adult mouse spleen cells and bone marrow hematopoietic stem cells. Upper Plots: BALB/c spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567653; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing CD150 expression or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative lymphocytes. Lower Plots: BALB/c mouse bone marrow cells were labeled with the BD IMag Mouse Hematopoietic Progenitor Enrichment Set Cat. No. 558451 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 . The final hematopoietic progenitor-enriched fraction was subsequently stained with APC Mouse Lineage Antibody Cocktail Cat. No. 558074 , BD Horizon BUV395 Rat Anti-Mouse CD41 Cat. No. 564056/565980 , FITC Hamster Anti-Mouse CD48 Cat. No. 557484 and BD Horizon PE-CF594 Rat Anti-Mouse CD150 SLAM antibodies. The bivariate pseudocolor density plot showing the coexpressed levels of CD150 versus CD48 by viable light scatter-gated cells Left Plot was further gated to show CD41 expression Right Plot on lineage-negative CD150+CD48- cells. Lineage-negative CD150+CD48-CD41- bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD150 SLAM expression by adult mouse spleen cells and bone marrow hematopoietic stem cells. Upper Plots: BALB/c spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567653; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing CD150 expression or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative lymphocytes. Lower Plots: BALB/c mouse bone marrow cells were labeled with the BD IMag Mouse Hematopoietic Progenitor Enrichment Set Cat. No. 558451 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 . The final hematopoietic progenitor-enriched fraction was subsequently stained with APC Mouse Lineage Antibody Cocktail Cat. No. 558074 , BD Horizon BUV395 Rat Anti-Mouse CD41 Cat. No. 564056/565980 , FITC Hamster Anti-Mouse CD48 Cat. No. 557484 and BD Horizon PE-CF594 Rat Anti-Mouse CD150 SLAM antibodies. The bivariate pseudocolor density plot showing the coexpressed levels of CD150 versus CD48 by viable light scatter-gated cells Left Plot was further gated to show CD41 expression Right Plot on lineage-negative CD150+CD48- cells. Lineage-negative CD150+CD48-CD41- bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD150 SLAM expression by adult mouse spleen cells and bone marrow hematopoietic stem cells. Upper Plots: BALB/c spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse CD150 SLAM antibody Cat. No. 567653; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing CD150 expression or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative lymphocytes. Lower Plots: BALB/c mouse bone marrow cells were labeled with the BD IMag Mouse Hematopoietic Progenitor Enrichment Set Cat. No. 558451 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 . The final hematopoietic progenitor-enriched fraction was subsequently stained with APC Mouse Lineage Antibody Cocktail Cat. No. 558074 , BD Horizon BUV395 Rat Anti-Mouse CD41 Cat. No. 564056/565980 , FITC Hamster Anti-Mouse CD48 Cat. No. 557484 and BD Horizon PE-CF594 Rat Anti-Mouse CD150 SLAM antibodies. The bivariate pseudocolor density plot showing the coexpressed levels of CD150 versus CD48 by viable light scatter-gated cells Left Plot was further gated to show CD41 expression Right Plot on lineage-negative CD150+CD48- cells. Lineage-negative CD150+CD48-CD41- bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Data shown on this Technical Data Sheet are not lot specific.