BD HorizonTM PE-CF594 Rat Anti-Mouse CD192 (CCR2)

Description

Multicolor flow cytometric analysis of CD192 CCR2 expression on viable Mouse splenic leukocytes. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Cat. No. 562309; Left Plots or BD Horizon PE-CF594 Rat Anti-Mouse CD192 CCR2 antibody Cat. No. 568804/568805; Right Plots at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD192 CCR2 expression on viable Mouse splenic leukocytes. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Cat. No. 562309; Left Plots or BD Horizon PE-CF594 Rat Anti-Mouse CD192 CCR2 antibody Cat. No. 568804/568805; Right Plots at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD192 CCR2 expression on viable Mouse splenic leukocytes. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Cat. No. 562309; Left Plots or BD Horizon PE-CF594 Rat Anti-Mouse CD192 CCR2 antibody Cat. No. 568804/568805; Right Plots at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.