
562847 ·
BDB Bioscience · Cat: 562847
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Description
Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 , and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Panel or BD Horizon PE-CF594 Rat Anti-Mouse CD40 antibody Cat. No. 562847; Right Panel . Two-color flow cytometric dot plots show the correlated expression of CD40 or Ig Isotype Control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 , and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Panel or BD Horizon PE-CF594 Rat Anti-Mouse CD40 antibody Cat. No. 562847; Right Panel . Two-color flow cytometric dot plots show the correlated expression of CD40 or Ig Isotype Control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 , and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Panel or BD Horizon PE-CF594 Rat Anti-Mouse CD40 antibody Cat. No. 562847; Right Panel . Two-color flow cytometric dot plots show the correlated expression of CD40 or Ig Isotype Control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 , and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Panel or BD Horizon PE-CF594 Rat Anti-Mouse CD40 antibody Cat. No. 562847; Right Panel . Two-color flow cytometric dot plots show the correlated expression of CD40 or Ig Isotype Control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 , and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Panel or BD Horizon PE-CF594 Rat Anti-Mouse CD40 antibody Cat. No. 562847; Right Panel . Two-color flow cytometric dot plots show the correlated expression of CD40 or Ig Isotype Control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD40 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 , and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; Left Panel or BD Horizon PE-CF594 Rat Anti-Mouse CD40 antibody Cat. No. 562847; Right Panel . Two-color flow cytometric dot plots show the correlated expression of CD40 or Ig Isotype Control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.