BD HorizonTM PE-CF594 Rat Anti-Mouse FoxP3

Description

Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302 or BD Horizon PE-CF594 Rat Anti-Mouse Foxp3 antibody Cat. No. 567373 at 0.25 mug/test. The pseudocolor bivariate density plots showing the correlated expression patterns of Foxp3 or Ig Isotype control staining versus Foxp3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302 or BD Horizon PE-CF594 Rat Anti-Mouse Foxp3 antibody Cat. No. 567373 at 0.25 mug/test. The pseudocolor bivariate density plots showing the correlated expression patterns of Foxp3 or Ig Isotype control staining versus Foxp3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302 or BD Horizon PE-CF594 Rat Anti-Mouse Foxp3 antibody Cat. No. 567373 at 0.25 mug/test. The pseudocolor bivariate density plots showing the correlated expression patterns of Foxp3 or Ig Isotype control staining versus Foxp3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302 or BD Horizon PE-CF594 Rat Anti-Mouse Foxp3 antibody Cat. No. 567373 at 0.25 mug/test. The pseudocolor bivariate density plots showing the correlated expression patterns of Foxp3 or Ig Isotype control staining versus Foxp3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302 or BD Horizon PE-CF594 Rat Anti-Mouse Foxp3 antibody Cat. No. 567373 at 0.25 mug/test. The pseudocolor bivariate density plots showing the correlated expression patterns of Foxp3 or Ig Isotype control staining versus Foxp3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 , then fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302 or BD Horizon PE-CF594 Rat Anti-Mouse Foxp3 antibody Cat. No. 567373 at 0.25 mug/test. The pseudocolor bivariate density plots showing the correlated expression patterns of Foxp3 or Ig Isotype control staining versus Foxp3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.