BD HorizonTM PE-CF594 Rat Anti-Mouse Ly-6C

Description

Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. Top Plots: C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Cat. No. 562309; Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse Ly-6C antibody Cat. No. 570053/570134; Right Plot at 0.5 mug/test. Bottom Plots: C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with BD Horizon BB515 Rat Anti-Mouse CD11b antibody Cat. No. 564454 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse Ly-6C antibody Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a Top Plots or CD11b Bottom Plots were derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. Top Plots: C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Cat. No. 562309; Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse Ly-6C antibody Cat. No. 570053/570134; Right Plot at 0.5 mug/test. Bottom Plots: C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with BD Horizon BB515 Rat Anti-Mouse CD11b antibody Cat. No. 564454 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse Ly-6C antibody Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a Top Plots or CD11b Bottom Plots were derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. Top Plots: C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Cat. No. 562309; Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse Ly-6C antibody Cat. No. 570053/570134; Right Plot at 0.5 mug/test. Bottom Plots: C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with BD Horizon BB515 Rat Anti-Mouse CD11b antibody Cat. No. 564454 and with either BD Horizon PE-CF594 Rat IgG1, kappa Isotype Control Left Plot or BD Horizon PE-CF594 Rat Anti-Mouse Ly-6C antibody Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a Top Plots or CD11b Bottom Plots were derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.