BD HorizonTM R718 Hamster Anti-Mouse CD48

Description

Flow cytometric analysis of CD48 on mouse splenocytes. Mouse splenic leukocytes from a Balb/c mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with no antibody for a Fluorescence-Minus-One FMO control; dashed line histogram or stained with BD Horizon R718 Hamster Anti-Mouse CD48 antibody Cat. No. 567301; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms showing CD48 expression or FMO control were derived from gated events with the forward and side light-scattering characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD48 on mouse splenocytes. Mouse splenic leukocytes from a Balb/c mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with no antibody for a Fluorescence-Minus-One FMO control; dashed line histogram or stained with BD Horizon R718 Hamster Anti-Mouse CD48 antibody Cat. No. 567301; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms showing CD48 expression or FMO control were derived from gated events with the forward and side light-scattering characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD48 on mouse splenocytes. Mouse splenic leukocytes from a Balb/c mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with no antibody for a Fluorescence-Minus-One FMO control; dashed line histogram or stained with BD Horizon R718 Hamster Anti-Mouse CD48 antibody Cat. No. 567301; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms showing CD48 expression or FMO control were derived from gated events with the forward and side light-scattering characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Data shown on this Technical Data Sheet are not lot specific.