BD HorizonTM R718 Hamster Anti-Mouse Notch4

Description

Multicolor flow cytometric analysis of Notch4 expression on viable Mouse splenic dendritic cells. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon R718 Hamster IgG1, kappa Isotype Control Cat. No. 566951; Left Plot or BD Horizon R718 Hamster Anti-Mouse Notch4 antibody Cat. No. 569817/569892; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The contour plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Notch4 expression on viable Mouse splenic dendritic cells. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon R718 Hamster IgG1, kappa Isotype Control Cat. No. 566951; Left Plot or BD Horizon R718 Hamster Anti-Mouse Notch4 antibody Cat. No. 569817/569892; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The contour plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Notch4 expression on viable Mouse splenic dendritic cells. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon R718 Hamster IgG1, kappa Isotype Control Cat. No. 566951; Left Plot or BD Horizon R718 Hamster Anti-Mouse Notch4 antibody Cat. No. 569817/569892; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The contour plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Notch4 expression on viable Mouse splenic dendritic cells. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon R718 Hamster IgG1, kappa Isotype Control Cat. No. 566951; Left Plot or BD Horizon R718 Hamster Anti-Mouse Notch4 antibody Cat. No. 569817/569892; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The contour plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Notch4 expression on viable Mouse splenic dendritic cells. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon R718 Hamster IgG1, kappa Isotype Control Cat. No. 566951; Left Plot or BD Horizon R718 Hamster Anti-Mouse Notch4 antibody Cat. No. 569817/569892; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The contour plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Notch4 expression on viable Mouse splenic dendritic cells. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD11c Cat. No. 562782 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon R718 Hamster IgG1, kappa Isotype Control Cat. No. 566951; Left Plot or BD Horizon R718 Hamster Anti-Mouse Notch4 antibody Cat. No. 569817/569892; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The contour plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.