BD HorizonTM R718 Mouse Anti-GATA3

Description

Flow cytometric analysis of GATA3 expression in Jurkat cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon R718 Mouse Anti-GATA3 antibody Cat. No. 567632/567633 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo software. Flow cytometric analysis of GATA3 expression in Jurkat cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon R718 Mouse Anti-GATA3 antibody Cat. No. 567632/567633 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of GATA3 expression in Jurkat cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon R718 Mouse Anti-GATA3 antibody Cat. No. 567632/567633 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo software.