BD HorizonTM R718 Mouse Anti-Human C3b/iC3b

Description

Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Left Plot; Isotype Control + Serum or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Right Plot; Anti-CD3 + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either BD Horizon R718 Mouse IgG1, Isotype Control Cat. No. 566928; dotted line histograms or with BD Horizon R718 Mouse Anti-Human C3b/iC3b antibody Cat. No. 568048/568049; solid line histograms . 7-Amino-Actinomycin D 7-AAD Cat. No. 559925 was added to cells right before analysis. The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Left Plot; Isotype Control + Serum or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Right Plot; Anti-CD3 + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either BD Horizon R718 Mouse IgG1, Isotype Control Cat. No. 566928; dotted line histograms or with BD Horizon R718 Mouse Anti-Human C3b/iC3b antibody Cat. No. 568048/568049; solid line histograms . 7-Amino-Actinomycin D 7-AAD Cat. No. 559925 was added to cells right before analysis. The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Left Plot; Isotype Control + Serum or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Right Plot; Anti-CD3 + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either BD Horizon R718 Mouse IgG1, Isotype Control Cat. No. 566928; dotted line histograms or with BD Horizon R718 Mouse Anti-Human C3b/iC3b antibody Cat. No. 568048/568049; solid line histograms . 7-Amino-Actinomycin D 7-AAD Cat. No. 559925 was added to cells right before analysis. The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Left Plot; Isotype Control + Serum or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Right Plot; Anti-CD3 + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either BD Horizon R718 Mouse IgG1, Isotype Control Cat. No. 566928; dotted line histograms or with BD Horizon R718 Mouse Anti-Human C3b/iC3b antibody Cat. No. 568048/568049; solid line histograms . 7-Amino-Actinomycin D 7-AAD Cat. No. 559925 was added to cells right before analysis. The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Left Plot; Isotype Control + Serum or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Right Plot; Anti-CD3 + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either BD Horizon R718 Mouse IgG1, Isotype Control Cat. No. 566928; dotted line histograms or with BD Horizon R718 Mouse Anti-Human C3b/iC3b antibody Cat. No. 568048/568049; solid line histograms . 7-Amino-Actinomycin D 7-AAD Cat. No. 559925 was added to cells right before analysis. The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Left Plot; Isotype Control + Serum or Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Right Plot; Anti-CD3 + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either BD Horizon R718 Mouse IgG1, Isotype Control Cat. No. 566928; dotted line histograms or with BD Horizon R718 Mouse Anti-Human C3b/iC3b antibody Cat. No. 568048/568049; solid line histograms . 7-Amino-Actinomycin D 7-AAD Cat. No. 559925 was added to cells right before analysis. The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.