BD HorizonTM R718 Mouse Anti-Human CD137

Description

Flow cytometric analysis of CD137 expression on stimulated human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD137 antibody Cat. No. 567086/567223; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expressed levels of CD137 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD137 expression on stimulated human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD137 antibody Cat. No. 567086/567223; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expressed levels of CD137 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD137 expression on stimulated human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD137 antibody Cat. No. 567086/567223; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expressed levels of CD137 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.