BD HorizonTM R718 Mouse Anti-Human CD152

Description

Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human CD152 antibody Cat. No. 567226/567070; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human CD152 antibody Cat. No. 567226/567070; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human CD152 antibody Cat. No. 567226/567070; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human CD152 antibody Cat. No. 567226/567070; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human CD152 antibody Cat. No. 567226/567070; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human CD152 antibody Cat. No. 567226/567070; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.