
567984 · 100 Tests
BDB Bioscience · Cat: 567984
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of CD1d expression on different human peripheral blood leucocyte populations . Whole blood was stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994; Bottom Plots and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human CD1d antibody Cat. No. 567984/567985; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of CD1d or Ig Isotype control staining versus side-light scatter SSC-A signals Top Plots or CD19 Bottom Plots were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD1d expression on different human peripheral blood leucocyte populations . Whole blood was stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994; Bottom Plots and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human CD1d antibody Cat. No. 567984/567985; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of CD1d or Ig Isotype control staining versus side-light scatter SSC-A signals Top Plots or CD19 Bottom Plots were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD1d expression on different human peripheral blood leucocyte populations . Whole blood was stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994; Bottom Plots and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human CD1d antibody Cat. No. 567984/567985; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of CD1d or Ig Isotype control staining versus side-light scatter SSC-A signals Top Plots or CD19 Bottom Plots were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.