BD HorizonTM R718 Mouse Anti-Human CD20

Description

Multicolor flow cytometric analysis of CD20 cytoplasmic domain expression in Human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; dashed line histogram or BD Horizon RY718 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 568050/568051; solid line histogram . The bivariate pseudocolor density plot showing the correlated expression of CD20 cytoplasmatic domain or Ig Isotype control staining vs side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD20 cytoplasmic domain expression in Human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; dashed line histogram or BD Horizon RY718 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 568050/568051; solid line histogram . The bivariate pseudocolor density plot showing the correlated expression of CD20 cytoplasmatic domain or Ig Isotype control staining vs side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD20 cytoplasmic domain expression in Human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; dashed line histogram or BD Horizon RY718 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 568050/568051; solid line histogram . The bivariate pseudocolor density plot showing the correlated expression of CD20 cytoplasmatic domain or Ig Isotype control staining vs side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD20 cytoplasmic domain expression in Human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; dashed line histogram or BD Horizon RY718 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 568050/568051; solid line histogram . The bivariate pseudocolor density plot showing the correlated expression of CD20 cytoplasmatic domain or Ig Isotype control staining vs side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD20 cytoplasmic domain expression in Human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; dashed line histogram or BD Horizon RY718 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 568050/568051; solid line histogram . The bivariate pseudocolor density plot showing the correlated expression of CD20 cytoplasmatic domain or Ig Isotype control staining vs side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD20 cytoplasmic domain expression in Human peripheral blood lymphocytes. Whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 for 10 min at 37C to lyse erythrocytes and fix the leucocytes in one step. The leucocytes were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 for 20 minutes. The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; dashed line histogram or BD Horizon RY718 Mouse Anti-Human CD20 cytoplasmic antibody Cat. No. 568050/568051; solid line histogram . The bivariate pseudocolor density plot showing the correlated expression of CD20 cytoplasmatic domain or Ig Isotype control staining vs side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.