BD HorizonTM R718 Mouse Anti-Human CD25

Description

Multicolor flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD25 antibody Cat. No. 567575/567576; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. CD25 expression on activated lymphocytes. Human PBMC were stimulated with PHA 3 days . Cells were stained with BD Horizon R718 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon R718 Mouse Anti-Human CD25 solid line histogram . Fluorescent histograms showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. BD LSRFortessa X-20 Cell Analyzer System and FlowJo software were used. Multicolor flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD25 antibody Cat. No. 567575/567576; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More CD25 expression on activated lymphocytes. Human PBMC were stimulated with PHA 3 days . Cells were stained with BD Horizon R718 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon R718 Mouse Anti-Human CD25 solid line histogram . Fluorescent histograms showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. BD LSRFortessa X-20 Cell Analyzer System and FlowJo software were used. Show More Multicolor flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD25 antibody Cat. No. 567575/567576; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. CD25 expression on activated lymphocytes. Human PBMC were stimulated with PHA 3 days . Cells were stained with BD Horizon R718 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon R718 Mouse Anti-Human CD25 solid line histogram . Fluorescent histograms showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. BD LSRFortessa X-20 Cell Analyzer System and FlowJo software were used.