BD HorizonTM R718 Mouse Anti-Human CD279 (PD-1)

Description

Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 563797/563798 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566974/566975; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 563797/563798 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566974/566975; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 563797/563798 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566974/566975; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 563797/563798 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566974/566975; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 563797/563798 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566974/566975; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 563797/563798 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 566974/566975; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color flow cytometric contour plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.