BD HorizonTM R718 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on human peripheral blood leucocyte populations. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD45RO antibodies Cat. No. 567536/567537; Right Plots . Erythrocytes were lysed with BD FACS Lysing Buffer Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the side and forward light-scattering characteristics of intact leucocyte populations. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig isotype control staining versus CD45RA was derived from gated events with the side and forward light-scattering characteristics of intact lymphocytes. Multiparameter flow cytometric analysis of CD45RO expression on human peripheral blood leucocyte populations. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD45RO antibodies Cat. No. 567536/567537; Right Plots . Erythrocytes were lysed with BD FACS Lysing Buffer Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the side and forward light-scattering characteristics of intact leucocyte populations. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig isotype control staining versus CD45RA was derived from gated events with the side and forward light-scattering characteristics of intact lymphocytes. Show More Multiparameter flow cytometric analysis of CD45RO expression on human peripheral blood leucocyte populations. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD45RO antibodies Cat. No. 567536/567537; Right Plots . Erythrocytes were lysed with BD FACS Lysing Buffer Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the side and forward light-scattering characteristics of intact leucocyte populations. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig isotype control staining versus CD45RA was derived from gated events with the side and forward light-scattering characteristics of intact lymphocytes.