
568069 · 100 Tests
BDB Bioscience · Cat: 568069
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD68 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon R718 Mouse IgG2b, kappa Isotype Control Cat. No. 566952; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD68 antibody Cat. No. 568069/568070; solid line histogram . The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD68 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon R718 Mouse IgG2b, kappa Isotype Control Cat. No. 566952; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD68 antibody Cat. No. 568069/568070; solid line histogram . The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD68 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon R718 Mouse IgG2b, kappa Isotype Control Cat. No. 566952; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD68 antibody Cat. No. 568069/568070; solid line histogram . The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.