BD HorizonTM R718 Mouse Anti-Human CD8

Description

Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426; Top Plots and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD8 antibody Cat. No. 569415/569416; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of CD8 or Ig Isotype control staining versus CD3 Top Plots or side-light scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426; Top Plots and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD8 antibody Cat. No. 569415/569416; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of CD8 or Ig Isotype control staining versus CD3 Top Plots or side-light scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426; Top Plots and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD8 antibody Cat. No. 569415/569416; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of CD8 or Ig Isotype control staining versus CD3 Top Plots or side-light scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426; Top Plots and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD8 antibody Cat. No. 569415/569416; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of CD8 or Ig Isotype control staining versus CD3 Top Plots or side-light scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426; Top Plots and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD8 antibody Cat. No. 569415/569416; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of CD8 or Ig Isotype control staining versus CD3 Top Plots or side-light scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426; Top Plots and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plots or BD Horizon R718 Mouse Anti-Human CD8 antibody Cat. No. 569415/569416; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of CD8 or Ig Isotype control staining versus CD3 Top Plots or side-light scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.