
567055 · 100 Tests
BDB Bioscience · Cat: 567055
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD80 expression on human Raji cells. Cells from the human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histogram or BD Horizon BV718 Mouse Anti-Human CD80 antibody Cat. No. 567055/ 567240; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD80 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD80 expression on human Raji cells. Cells from the human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histogram or BD Horizon BV718 Mouse Anti-Human CD80 antibody Cat. No. 567055/ 567240; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD80 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD80 expression on human Raji cells. Cells from the human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; dashed line histogram or BD Horizon BV718 Mouse Anti-Human CD80 antibody Cat. No. 567055/ 567240; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD80 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.