BD HorizonTM R718 Mouse Anti-Human CD80 (B7-1)

Description

Flow cytometric analysis of CD80 B7-1 expression on human Raji cells. Cells from the human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No. 566928; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD80 B7-1 antibody Cat. No. 567442/567441 solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD80 B7-1 expression on human Raji cells. Cells from the human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No. 566928; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD80 B7-1 antibody Cat. No. 567442/567441 solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD80 B7-1 expression on human Raji cells. Cells from the human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No. 566928; dashed line histogram or BD Horizon R718 Mouse Anti-Human CD80 B7-1 antibody Cat. No. 567442/567441 solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.