BD HorizonTM R718 Mouse Anti-Human FoxP3

Description

Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724; Upper Plots and FITC Anti-Human CD25 Cat. No. 555431/560990; Lower Plots antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human FoxP3 antibody Cat No. 567243/567067; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of FoxP3 versus either CD4 or CD25 or Ig Isotype control staining were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724; Upper Plots and FITC Anti-Human CD25 Cat. No. 555431/560990; Lower Plots antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human FoxP3 antibody Cat No. 567243/567067; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of FoxP3 versus either CD4 or CD25 or Ig Isotype control staining were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724; Upper Plots and FITC Anti-Human CD25 Cat. No. 555431/560990; Lower Plots antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human FoxP3 antibody Cat No. 567243/567067; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of FoxP3 versus either CD4 or CD25 or Ig Isotype control staining were derived from gated events with the light scattering characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.