BD HorizonTM R718 Mouse Anti-Human Granzyme K

Description

Two-color flow cytometric analysis of Granzyme K expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme K antibody Cat. No. 570343/570413; Right Plot . The pseudocolor density plot showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of Granzyme K expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme K antibody Cat. No. 570343/570413; Right Plot . The pseudocolor density plot showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of Granzyme K expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 567089; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme K antibody Cat. No. 570343/570413; Right Plot . The pseudocolor density plot showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.