BD HorizonTM R718 Mouse Anti-Human IDO1

Description

Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMC . PBMC were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer Cat. 554655 and washed and permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Mouse Anti-Human CD11b Cat. No. 561001 and BD Horizon R718 Mouse Anti-Human IDO1 Cat. No. 568016/568017 antibodies. The bivariate pseudocolor density plots showing the correlated expression of IDO1 versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer and FlowJo software. Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMC . PBMC were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer Cat. 554655 and washed and permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Mouse Anti-Human CD11b Cat. No. 561001 and BD Horizon R718 Mouse Anti-Human IDO1 Cat. No. 568016/568017 antibodies. The bivariate pseudocolor density plots showing the correlated expression of IDO1 versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer and FlowJo software. Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMC . PBMC were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer Cat. 554655 and washed and permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Mouse Anti-Human CD11b Cat. No. 561001 and BD Horizon R718 Mouse Anti-Human IDO1 Cat. No. 568016/568017 antibodies. The bivariate pseudocolor density plots showing the correlated expression of IDO1 versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer and FlowJo software. Show More Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMC . PBMC were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer Cat. 554655 and washed and permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Mouse Anti-Human CD11b Cat. No. 561001 and BD Horizon R718 Mouse Anti-Human IDO1 Cat. No. 568016/568017 antibodies. The bivariate pseudocolor density plots showing the correlated expression of IDO1 versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer and FlowJo software. Show More Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMC . PBMC were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer Cat. 554655 and washed and permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Mouse Anti-Human CD11b Cat. No. 561001 and BD Horizon R718 Mouse Anti-Human IDO1 Cat. No. 568016/568017 antibodies. The bivariate pseudocolor density plots showing the correlated expression of IDO1 versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer and FlowJo software. Multicolor flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMC . PBMC were cultured in complete tissue culture medium without Left Plot or with Right Plot lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer Cat. 554655 and washed and permeabilized using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Mouse Anti-Human CD11b Cat. No. 561001 and BD Horizon R718 Mouse Anti-Human IDO1 Cat. No. 568016/568017 antibodies. The bivariate pseudocolor density plots showing the correlated expression of IDO1 versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer and FlowJo software.