BD HorizonTM R718 Mouse Anti-Human IgD

Description

Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD HorizonBV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human IgD antibody Cat. No. 567993/567994; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD HorizonBV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human IgD antibody Cat. No. 567993/567994; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD HorizonBV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human IgD antibody Cat. No. 567993/567994; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD HorizonBV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human IgD antibody Cat. No. 567993/567994; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD HorizonBV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human IgD antibody Cat. No. 567993/567994; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with BD HorizonBV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; Left Plot or BD Horizon R718 Mouse Anti-Human IgD antibody Cat. No. 567993/567994; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.