BD HorizonTM R718 Mouse Anti-Human IgM

Description

Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No.566928; Left Plot or BD Horizon R718 Mouse Anti-Human IgM antibody Cat. No. 567639/567640; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No.566928; Left Plot or BD Horizon R718 Mouse Anti-Human IgM antibody Cat. No. 567639/567640; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No.566928; Left Plot or BD Horizon R718 Mouse Anti-Human IgM antibody Cat. No. 567639/567640; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No.566928; Left Plot or BD Horizon R718 Mouse Anti-Human IgM antibody Cat. No. 567639/567640; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No.566928; Left Plot or BD Horizon R718 Mouse Anti-Human IgM antibody Cat. No. 567639/567640; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No.566928; Left Plot or BD Horizon R718 Mouse Anti-Human IgM antibody Cat. No. 567639/567640; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software.