BD HorizonTM R718 Mouse Anti-Human NCAM-1 (CD56)

Description

Two-color flow cytometric analysis of NCAM-1 CD56 expression by fixed and permeabilized Human peripheral blood lymphocytes. Erythrocytes were lysed and leukocytes were fixed and permeabilized in a whole blood sample using 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C followed by BD Phosflow Perm Buffer III Cat. No. 558050; 30 min, on ice . The leukocytes were then stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 and BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 Cat. No. 569802/569876 antibodies. The bivariate pseudocolor density plot showing the correlated expression of CD3 versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of NCAM-1 CD56 expression on live-stained Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 and BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 Cat. No. 569802/569876 antibodies. Erythrocytes were lysed with BD FACS Lysing Solution Cat. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD3 versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of NCAM-1 CD56 expression by fixed and permeabilized Human peripheral blood lymphocytes. Erythrocytes were lysed and leukocytes were fixed and permeabilized in a whole blood sample using 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C followed by BD Phosflow Perm Buffer III Cat. No. 558050; 30 min, on ice . The leukocytes were then stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 and BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 Cat. No. 569802/569876 antibodies. The bivariate pseudocolor density plot showing the correlated expression of CD3 versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of NCAM-1 CD56 expression on live-stained Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 and BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 Cat. No. 569802/569876 antibodies. Erythrocytes were lysed with BD FACS Lysing Solution Cat. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD3 versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of NCAM-1 CD56 expression by fixed and permeabilized Human peripheral blood lymphocytes. Erythrocytes were lysed and leukocytes were fixed and permeabilized in a whole blood sample using 1X BD Phosflow Lyse/Fix Buffer Cat. No. 558049; 10 min, 37 C followed by BD Phosflow Perm Buffer III Cat. No. 558050; 30 min, on ice . The leukocytes were then stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 and BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 Cat. No. 569802/569876 antibodies. The bivariate pseudocolor density plot showing the correlated expression of CD3 versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of NCAM-1 CD56 expression on live-stained Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426/562427 and BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 Cat. No. 569802/569876 antibodies. Erythrocytes were lysed with BD FACS Lysing Solution Cat. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD3 versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software.