BD HorizonTM R718 Mouse Anti-Human TCRalphabeta

Description

Multicolor flow cytometric analysis of TCRalphabeta expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 659477 , FITC Mouse Anti-Human CD3 antibody Cat. No. 555916/555332/561807/561806 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human TCRalphabeta antibody Cat. No. 567676/567677; Right Plots . Erythrocytes were lysed with BD FACSLyse Lysing Buffer Cat. No. 349202 . Flow cytometric analysis was performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD19 versus TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD3 versus TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of TCRalphabeta expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 659477 , FITC Mouse Anti-Human CD3 antibody Cat. No. 555916/555332/561807/561806 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human TCRalphabeta antibody Cat. No. 567676/567677; Right Plots . Erythrocytes were lysed with BD FACSLyse Lysing Buffer Cat. No. 349202 . Flow cytometric analysis was performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD19 versus TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD3 versus TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of TCRalphabeta expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 659477 , FITC Mouse Anti-Human CD3 antibody Cat. No. 555916/555332/561807/561806 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human TCRalphabeta antibody Cat. No. 567676/567677; Right Plots . Erythrocytes were lysed with BD FACSLyse Lysing Buffer Cat. No. 349202 . Flow cytometric analysis was performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD19 versus TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of CD3 versus TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.