BD HorizonTM R718 Mouse Anti-Human TIM-3 (CD366)

Description

Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555516; Lower Plots and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 567557/567558; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Upper Plots: The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Lower Plots: The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555516; Lower Plots and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 567557/567558; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Upper Plots: The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Lower Plots: The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Show More Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555516; Lower Plots and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plots or BD Horizon R718 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 567557/567558; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Upper Plots: The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Lower Plots: The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes.