BD HorizonTM R718 Mouse Anti-Mouse CD159a (NKG2AB6)

Description

Multicolor flow cytometric analysis of Mouse strain-specific expression of CD159a NKG2AB6 on splenic NK cells. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD49b antibody Cat. No. 563063 and with either BD Horizon R718 Mouse IgG2b, kappa Isotype Control Cat. No. 566952; Left Plots or BD Horizon R718 Mouse Anti-Mouse CD159a NKG2AB6 antibody Cat. No. 570005/570086; Right Plots at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD159a NKG2AB6 or Ig Isotype control staining versus CD49b were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Mouse strain-specific expression of CD159a NKG2AB6 on splenic NK cells. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD49b antibody Cat. No. 563063 and with either BD Horizon R718 Mouse IgG2b, kappa Isotype Control Cat. No. 566952; Left Plots or BD Horizon R718 Mouse Anti-Mouse CD159a NKG2AB6 antibody Cat. No. 570005/570086; Right Plots at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD159a NKG2AB6 or Ig Isotype control staining versus CD49b were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Mouse strain-specific expression of CD159a NKG2AB6 on splenic NK cells. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD49b antibody Cat. No. 563063 and with either BD Horizon R718 Mouse IgG2b, kappa Isotype Control Cat. No. 566952; Left Plots or BD Horizon R718 Mouse Anti-Mouse CD159a NKG2AB6 antibody Cat. No. 570005/570086; Right Plots at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD159a NKG2AB6 or Ig Isotype control staining versus CD49b were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.