BD HorizonTM R718 Mouse Anti-Mouse CD272 (BTLA)

Description

Multicolor flow cytometric analysis of CD272 BTLA expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD272 BTLA antibody Cat. No. 569840/569916; Right Plot at 1 mug /test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD272 BTLA or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD272 BTLA expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD272 BTLA antibody Cat. No. 569840/569916; Right Plot at 1 mug /test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD272 BTLA or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD272 BTLA expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD272 BTLA antibody Cat. No. 569840/569916; Right Plot at 1 mug /test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD272 BTLA or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD272 BTLA expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD272 BTLA antibody Cat. No. 569840/569916; Right Plot at 1 mug /test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD272 BTLA or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD272 BTLA expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD272 BTLA antibody Cat. No. 569840/569916; Right Plot at 1 mug /test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD272 BTLA or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD272 BTLA expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD272 BTLA antibody Cat. No. 569840/569916; Right Plot at 1 mug /test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD272 BTLA or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.