BD HorizonTM R718 Mouse Anti-Mouse CD369 (CLEC7A)

Description

Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peritoneal exudate cells PEC . BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569951/569956; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus F4/80 was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD369 CLEC7A expression on Mouse peripheral blood leukocytes PBL . BALB/c Mouse peripheral blood leukocytes PBL were stained with PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569951/569956; Right Plot at 1 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peritoneal exudate cells PEC . BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569951/569956; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus F4/80 was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD369 CLEC7A expression on Mouse peripheral blood leukocytes PBL . BALB/c Mouse peripheral blood leukocytes PBL were stained with PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569951/569956; Right Plot at 1 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peritoneal exudate cells PEC . BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569951/569956; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus F4/80 was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD369 CLEC7A expression on Mouse peripheral blood leukocytes PBL . BALB/c Mouse peripheral blood leukocytes PBL were stained with PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 567470; Left Plot or BD Horizon R718 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569951/569956; Right Plot at 1 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.