BD HorizonTM R718 Mouse Anti-Mouse CD45.1

Description

Flow cytometric analysis of CD45.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse Left Plot and SJL/J Right Plot mouse were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse CD45.1 antibody Cat. No. 567297; solid line histograms at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse Left Plot and SJL/J Right Plot mouse were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse CD45.1 antibody Cat. No. 567297; solid line histograms at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse Left Plot and SJL/J Right Plot mouse were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse CD45.1 antibody Cat. No. 567297; solid line histograms at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse Left Plot and SJL/J Right Plot mouse were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse CD45.1 antibody Cat. No. 567297; solid line histograms at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse Left Plot and SJL/J Right Plot mouse were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse CD45.1 antibody Cat. No. 567297; solid line histograms at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse Left Plot and SJL/J Right Plot mouse were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse CD45.1 antibody Cat. No. 567297; solid line histograms at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.