BD HorizonTM R718 Mouse Anti-Mouse H-2Kb

Description

Flow cytometric analysis of H-2Kb expression on mouse splenocytes. Splenic leucocytes from a BALB/c Left Figure or a C57BL/6 Right Figure mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or with BD Horizon R718 Mouse Anti-Mouse H-2Kb antibody Cat. No. 567645; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescence histograms showing H-2Kb or Ig Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of H-2Kb expression on mouse splenocytes. Splenic leucocytes from a BALB/c Left Figure or a C57BL/6 Right Figure mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or with BD Horizon R718 Mouse Anti-Mouse H-2Kb antibody Cat. No. 567645; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescence histograms showing H-2Kb or Ig Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of H-2Kb expression on mouse splenocytes. Splenic leucocytes from a BALB/c Left Figure or a C57BL/6 Right Figure mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or with BD Horizon R718 Mouse Anti-Mouse H-2Kb antibody Cat. No. 567645; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescence histograms showing H-2Kb or Ig Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of H-2Kb expression on mouse splenocytes. Splenic leucocytes from a BALB/c Left Figure or a C57BL/6 Right Figure mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or with BD Horizon R718 Mouse Anti-Mouse H-2Kb antibody Cat. No. 567645; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescence histograms showing H-2Kb or Ig Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of H-2Kb expression on mouse splenocytes. Splenic leucocytes from a BALB/c Left Figure or a C57BL/6 Right Figure mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or with BD Horizon R718 Mouse Anti-Mouse H-2Kb antibody Cat. No. 567645; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescence histograms showing H-2Kb or Ig Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of H-2Kb expression on mouse splenocytes. Splenic leucocytes from a BALB/c Left Figure or a C57BL/6 Right Figure mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or with BD Horizon R718 Mouse Anti-Mouse H-2Kb antibody Cat. No. 567645; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescence histograms showing H-2Kb or Ig Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.