BD HorizonTM R718 Mouse Anti-Mouse H-2Kd

Description

Flow cytometric analysis of H-2Kd expression on viable BALB/c Mouse splenic leucocytes. SJL Left Figure and BALB/c Right Figure Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse H-2Kd antibody Cat. No. 569556/569557; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing H-2Kd expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kd expression on viable BALB/c Mouse splenic leucocytes. SJL Left Figure and BALB/c Right Figure Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse H-2Kd antibody Cat. No. 569556/569557; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing H-2Kd expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kd expression on viable BALB/c Mouse splenic leucocytes. SJL Left Figure and BALB/c Right Figure Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse H-2Kd antibody Cat. No. 569556/569557; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing H-2Kd expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2Kd expression on viable BALB/c Mouse splenic leucocytes. SJL Left Figure and BALB/c Right Figure Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse H-2Kd antibody Cat. No. 569556/569557; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing H-2Kd expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2Kd expression on viable BALB/c Mouse splenic leucocytes. SJL Left Figure and BALB/c Right Figure Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse H-2Kd antibody Cat. No. 569556/569557; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing H-2Kd expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kd expression on viable BALB/c Mouse splenic leucocytes. SJL Left Figure and BALB/c Right Figure Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949; dashed line histograms or BD Horizon R718 Mouse Anti-Mouse H-2Kd antibody Cat. No. 569556/569557; solid line histograms at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing H-2Kd expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.