BD HorizonTM R718 Mouse Anti-Mouse NK-1.1

Description

Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949, Left Plot or BD Horizon R718 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 567360; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression patterns of NK1.1 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949, Left Plot or BD Horizon R718 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 567360; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression patterns of NK1.1 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949, Left Plot or BD Horizon R718 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 567360; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression patterns of NK1.1 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949, Left Plot or BD Horizon R718 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 567360; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression patterns of NK1.1 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949, Left Plot or BD Horizon R718 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 567360; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression patterns of NK1.1 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. Splenic leucocytes from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949, Left Plot or BD Horizon R718 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 567360; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression patterns of NK1.1 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software.