BD HorizonTM R718 Mouse Anti-Mouse RORgammat

Description

Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and BD Horizon BV510 Rat Anti-Mouse CD8a Cat. No. 563068 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949 or BD Horizon R718 Mouse Anti-Mouse RORgammat antibody Cat. No. 567362 0.06 ug/test. The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histograms or CD4+ CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and BD Horizon BV510 Rat Anti-Mouse CD8a Cat. No. 563068 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949 or BD Horizon R718 Mouse Anti-Mouse RORgammat antibody Cat. No. 567362 0.06 ug/test. The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histograms or CD4+ CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and BD Horizon BV510 Rat Anti-Mouse CD8a Cat. No. 563068 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949 or BD Horizon R718 Mouse Anti-Mouse RORgammat antibody Cat. No. 567362 0.06 ug/test. The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histograms or CD4+ CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and BD Horizon BV510 Rat Anti-Mouse CD8a Cat. No. 563068 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949 or BD Horizon R718 Mouse Anti-Mouse RORgammat antibody Cat. No. 567362 0.06 ug/test. The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histograms or CD4+ CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and BD Horizon BV510 Rat Anti-Mouse CD8a Cat. No. 563068 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949 or BD Horizon R718 Mouse Anti-Mouse RORgammat antibody Cat. No. 567362 0.06 ug/test. The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histograms or CD4+ CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and BD Horizon BV510 Rat Anti-Mouse CD8a Cat. No. 563068 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon R718 Mouse IgG2a, kappa Isotype Control Cat. No. 566949 or BD Horizon R718 Mouse Anti-Mouse RORgammat antibody Cat. No. 567362 0.06 ug/test. The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histograms or CD4+ CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.